Bovine POLR2J ELISA KIT

Product Name:Bovine POLR2J ELISA KIT
Packing:96T

Catalog No.:ELI-36300b

Gene Name:Bovine POLR2J

Detect Range:0.781-50ng/ml

Sensitivity:0.469ng/ml

Target Protein Name:Bovine POLR2J

Alternative Name:POLR2J,Bovine POLR2J
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine POLR2J ELISA KIT allows for the in vitro quantitative determination of Bovine POLR2J concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine POLR2J ELISA KIT has been pre-coated with an Bovine POLR2J antibody specific to Bovine POLR2J .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine POLR2J and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine POLR2J, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine POLR2J in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!