Bovine Progesterone, PROG ELISA Kit
FOR RESEARCH USE ONLY!
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Bovine PROG ELISA KIT allows for the in vitro quantitative determination of Bovine progesterone,PROG concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Progesterone, During the reaction, Progesterone in the sample or standard competes with a fixed amount of biotin-labeled Progesterone for sites on a pre-coated Monoclonal antibody specific to Progesterone. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Progesterone in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 1.56-100 ng/ml
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