Bovine Prostaglandin F2 alpha, PGF2a ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Prostaglandin F2alpha, 8-iso-PGF2α, Prostaglandin F2α, PGF2α, dinoprost

Search name
Bovine PGF2a ELISA Kit, Bovine Prostaglandin F2 alpha ELISA Kit, Bovine 8-iso-PGF2α ELISA Kit, Bovine Prostaglandin F2α
ELISA Kit, Bovine PGF2αELISA Kit, Bovine dinoprost ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Prostaglandin F2alpha concentrations in serum, Plasma,
Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin F2alpha. Standards or samples are
then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Prostaglandin
F2alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate
solution is added to each well. Only those wells that contain Prostaglandin F2alpha, biotin-conjugated antibody and enzyme-conjugated
Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color
change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Prostaglandin F2alpha in the
samples is then determined by comparing the O.D. of the samples to the standard curve.

PDF manual download