Bovine FAM19A5 ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  FAM19A5

Protein Name  protein FAM19A5

Alternative Name

FAM19A5,protein FAM19A5; QLLK5208; TAFA-5; TAFA5; UNQ5208; TAFA protein 5; chemokine-like protein TAFA-5; family with sequence similarity 19 (chemokine (C-C motif)-like), member A5; family with sequence similarity 19 member A5, C-C motif chemokine lik

Intended use

Bovine FAM19A5 ELISA KIT allows for the in vitro quantitative determination of FAM19A5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Test principle      

The microtiter plate provided in Bovine FAM19A5 ELISA KIT has been pre-coated with an FAM19A5 antibody specific to FAM19A5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FAM19A5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FAM19A5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FAM19A5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20