Bovine Protein kinase C beta type, PRKCB ELISA KIT

Product Name:Bovine Protein kinase C beta type, PRKCB ELISA KIT
Packing:96T

Catalog No.:ELI-47859b

Gene Name:Bovine Protein kinase C beta type, PRKCB

Detect Range:31.2-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Bovine Protein kinase C beta type, PRKCB

Alternative Name:PRKCB,Bovine Protein kinase C beta type, PRKCB
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Protein kinase C beta type, PRKCB ELISA KIT allows for the in vitro quantitative determination of Bovine Protein kinase C beta type, PRKCB concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Protein kinase C beta type, PRKCB ELISA KIT has been pre-coated with an Bovine Protein kinase C beta type, PRKCB antibody specific to Bovine Protein kinase C beta type, PRKCB .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Protein kinase C beta type, PRKCB and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Protein kinase C beta type, PRKCB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Protein kinase C beta type, PRKCB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!