Bovine PTPRN ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name PTPRN
Protein Name Receptor-type tyrosine-protein phosphatase-like N
Alternative Name
Ptprn,IA 2,IA-2; IA-2/PTP; IA2; ICA512; R-PTP-N; receptor-type tyrosine-protein phosphatase-like N; ICA 512; PTP IA-2; insulinoma-associated tyrosine-phosphatase-like protein; islet cell antigen 2; islet cell antigen 512; islet cell autoantigen 3; protein tyrosine phosphatase-like N; protein tyrosine phosphatase, receptor type N
Intended use
Bovine PTPRN ELISA KIT allows for the in vitro quantitative determination of PTPRN concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Reagent | Quantity |
Assay plate | 1 |
Standard | 2 |
Sample Diluent | 1 × 20mL |
Assay Diluent A | 1 × 10mL |
Assay Diluent B | 1 × 10mL |
Detection Reagent A | 1 × 120μL |
Detection Reagent B | 1 × 120μL |
Wash Buffer(25 x concentrate) | 1 × 30mL |
Substrate | 1 × 10mL |
Stop Solution | 1 × 10mL |
Plate sealer | 5 |
Test principle
The microtiter plate provided in Bovine PTPRN ELISA KIT has been pre-coated with an PTPRN antibody specific to PTPRN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PTPRN and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PTPRN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PTPRN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20