Bovine Retinoid isomerohydrolase, RPE65 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

RPE65,BCO3; LCA2; RP20; mRPE65; rd12; sRPE65; Retinoid isomerohydrolase,BCO family member 3; RBP-binding membrane protein; all-trans-retinyl-palmitate hydrolase; p63; retinal pigment epithelium-specific 65 kDa protein; retinitis pigmentosa 20 autosomal recessive; retinol isomerase; retinal pigment epithelium-specific protein 65kDa

Search name

Bovine RPE65 ELISA KIT ,Bovine BCO3 ELISA KIT ,Bovine LCA2 ELISA KIT ,Bovine RP20 ELISA KIT ,Bovine mRPE65 ELISA KIT ,Bovine rd12 ELISA KIT ,Bovine sRPE65 ELISA KIT ,Bovine Retinoid isomerohydrolase ELISA KIT ,Bovine BCO family member 3 ELISA KIT ,Bovine RBP-binding membrane protein ELISA KIT ,Bovine all-trans-retinyl-palmitate hydrolase ELISA KIT ,Bovine p63 ELISA KIT ,Bovine retinal pigment epithelium-specific 65 kDa protein ELISA KIT ,Bovine retinitis pigmentosa 20 autosomal recessive ELISA KIT ,Bovine retinol isomerase ELISA KIT ,Bovine retinal pigment epithelium-specific protein 65kDa ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of bovine RPE65 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to RPE65. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RPE65 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RPE65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RPE65 in the samples is then determined by comparing the O.D. of the samples to the standard curve.