Bovine SFN (Stratifin) ELISA KIT

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Bovine Sfn ELISA KIT ,Bovine stratifin ELISA KIT ,Bovine Mkrn3 ELISA KIT ,Bovine YWHAS ELISA KIT ,Bovine 14-3-3 sigma ELISA KIT ,Bovine epithelial cell marker protein 1 ELISA KIT

Intended use

Bovine SFN ELISA kit allows for the in vitro quantitative determination of Bovine SFN concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Bovine SFN ELISA kit has been pre-coated with an antibody specific to SFN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SFN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SFN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SFN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Bovine SFN ELISA kit Manual Request by email:[email protected]

Order Bovine SFN ELISA kit by email:[email protected]