Bovine Squalene synthase, FDFT1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
FDFT1,squalene synthase,DGPT; ERG9; SQS; FPP:FPP farnesyltransferase; presqualene-di-diphosphate synthase; squalene synthetase; farnesyl-diphosphate farnesyltransferase 1
Search name
Bovine FDFT1 ELISA KIT ,Bovine squalene synthase ELISA KIT ,Bovine DGPT ELISA KIT ,Bovine ERG9 ELISA KIT ,Bovine SQS ELISA KIT ,Bovine FPP ELISA KIT ,Bovine FPP farnesyltransferase ELISA KIT ,Bovine presqualene-di-diphosphate synthase ELISA KIT ,Bovine squalene synthetase ELISA KIT ,Bovine farnesyl-diphosphate farnesyltransferase 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Squalene synthase, FDFT1 beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bovine Squalene synthase, FDFT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Bovine Squalene synthase, FDFT1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Squalene synthase, FDFT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Bovine Squalene synthase, FDFT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.