Bovine Signal transducer and activator of transcription 3, STAT3 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Signal transducer and activator of transcription 3,Acute-phase response factor, STAT3,APRF; HIES; DNA-binding protein APRF; acute-phase response factor; signal transducer and activator of transcription 3; signal transducer and activator of transcription 3 ,acute-phase response factor,

Search name

Bovine Signal transducer and activator of transcription 3 ELISA KIT ,Bovine Acute-phase response factor ELISA KIT ,Bovine  STAT3 ELISA KIT ,Bovine APRF ELISA KIT ,Bovine HIES ELISA KIT ,Bovine DNA-binding protein APRF ELISA KIT ,Bovine acute-phase response factor ELISA KIT ,Bovine signal transducer and activator of transcription 3 ELISA KIT ,Bovine signal transducer and activator of transcription 3  ELISA KIT ,Bovine acute-phase response factor ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of bovine Signal transducer and activator of transcription 3 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal transducer and activator of transcription 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Signal transducer and activator of transcription 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Signal transducer and activator of transcription 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Signal transducer and activator of transcription 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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