Bovine Syntaxin- 1A,STX1A ELISA KIT

Bovine Syntaxin- 1A, STX1A ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

STX1A,Syntaxin-1A,syntaxin, HPC-1; P35-1; STX1; SYN1A; neuron-specific antigen HPC-1; syntaxin 1A,Stx1a,Sap,Neuron-specific antigen HPC-1;Synaptotagmin-associated 35 kDa protein,P35A

Search name

Bovine STX1A ELISA KIT ,Bovine Syntaxin-1A ELISA KIT ,Bovine syntaxin ELISA KIT ,Bovine HPC-1 ELISA KIT ,Bovine P35-1 ELISA KIT ,Bovine STX1 ELISA KIT ,Bovine SYN1A ELISA KIT ,Bovine neuron-specific antigen HPC-1 ELISA KIT ,Bovine syntaxin 1A ELISA KIT ,Bovine Sap ELISA KIT ,Bovine Neuron-specific antigen HPC-1;Synaptotagmin-associated 35 kDa protein ELISA KIT ,Bovine P35A ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine Syntaxin- 1A, STX1A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to STX1A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for STX1A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain STX1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of STX1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.