Bovine Thromboxane A2, TX-A2 ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Thromboxane A2,TX-A2,

Search name
Bovine Thromboxane A2 ELISA Kit,Bovine TX-A2 ELISA Kit,Thromboxane A2 ELISA Kit,TX-A2 ELISA Kit,ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of thromboxane a2,Thromboxane A2 concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Thromboxane A2, During the reaction, Thromboxane A2 in the sample or standard competes with
a fixed amount of biotin-labeled Thromboxane A2 for sites on a pre-coated Monoclonal antibody specific to Thromboxane A2. Excess
conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is
added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450
nm ± 2 nm. The concentration of Thromboxane A2 in the samples is then determined by comparing the O.D. of the samples to the
standard curve.

PDF manual download