Bovine Toll-like receptor 4, TLR4 ELISA Kit
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Toll-like receptor 4, TLR4,LPS,hToll,CD284,ARMD10; TLR-4; TOLL; homolog of Drosophila toll;
Bovine Toll-like receptor 4 ELISA KIT ,Bovine TLR4 ELISA KIT ,Bovine LPS ELISA KIT ,Bovine hToll ELISA KIT ,Bovine CD284 ELISA KIT ,Bovine ARMD10 ELISA KIT ,Bovine TLR-4 ELISA KIT ,Bovine TOLL ELISA KIT ,Bovine homolog of Drosophila toll ELISA KIT, Bovine Toll4 ELISA Kit
This immunoassay kit allows for the in vitro quantitative determination of Bovine Toll-like receptor 4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll-like receptor 4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Toll-like receptor 4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Toll-like receptor 4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Toll-like receptor 4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.