Bovine Toll-like receptor 4, TLR4 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Toll-like receptor 4, TLR4,LPS,hToll,CD284,ARMD10; TLR-4; TOLL; homolog of Drosophila toll;

Search name

Bovine Toll-like receptor 4 ELISA KIT ,Bovine TLR4 ELISA KIT ,Bovine LPS ELISA KIT ,Bovine hToll ELISA KIT ,Bovine CD284 ELISA KIT ,Bovine ARMD10 ELISA KIT ,Bovine TLR-4 ELISA KIT ,Bovine TOLL ELISA KIT ,Bovine homolog of Drosophila toll ELISA KIT, Bovine Toll4 ELISA Kit

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine Toll-like receptor 4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll-like receptor 4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Toll-like receptor 4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Toll-like receptor 4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Toll-like receptor 4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.