Bovine Tribbles homolog 3, TRIB3 ELISA KIT
Product Name:Bovine Tribbles homolog 3, TRIB3 ELISA KIT
Packing:96T
Catalog No.:ELI-45762b
Gene Name:Bovine Tribbles homolog 3, TRIB3
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Bovine Tribbles homolog 3, TRIB3
Alternative Name:TRIB3,Bovine Tribbles homolog 3, TRIB3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Tribbles homolog 3, TRIB3 ELISA KIT allows for the in vitro quantitative determination of Bovine Tribbles homolog 3, TRIB3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Tribbles homolog 3, TRIB3 ELISA KIT has been pre-coated with an Bovine Tribbles homolog 3, TRIB3 antibody specific to Bovine Tribbles homolog 3, TRIB3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Tribbles homolog 3, TRIB3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Tribbles homolog 3, TRIB3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Tribbles homolog 3, TRIB3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!