Bovine Ubiquitin- conjugating enzyme E2 K, UBE2K ELISA KIT

Product Name:Bovine Ubiquitin- conjugating enzyme E2 K, UBE2K ELISA KIT
Packing:96T

Catalog No.:ELI-36926b

Gene Name:Bovine Ubiquitin-conjugating enzyme E2 K, UBE2K

Detect Range:15.625-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Bovine Ubiquitin-conjugating enzyme E2 K, UBE2K

Alternative Name:UBE2K,Bovine Ubiquitin-conjugating enzyme E2 K, UBE2K
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Ubiquitin- conjugating enzyme E2 K, UBE2K ELISA KIT allows for the in vitro quantitative determination of Bovine Ubiquitin-conjugating enzyme E2 K, UBE2K concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Ubiquitin- conjugating enzyme E2 K, UBE2K ELISA KIT has been pre-coated with an Bovine Ubiquitin-conjugating enzyme E2 K, UBE2K antibody specific to Bovine Ubiquitin-conjugating enzyme E2 K, UBE2K .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Ubiquitin-conjugating enzyme E2 K, UBE2K and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Ubiquitin-conjugating enzyme E2 K, UBE2K, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Ubiquitin-conjugating enzyme E2 K, UBE2K in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!