Bovine Uricase, UOX ELISA KIT

Product Name:Bovine Uricase, UOX ELISA KIT
Packing:96T

Catalog No.:ELI-51734b

Gene Name:Bovine Uricase, UOX

Detect Range:0.313-20ng/ml

Sensitivity:0.188ng/ml

Target Protein Name:Bovine Uricase, UOX

Alternative Name:UOX,Bovine Uricase, UOX
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Uricase, UOX ELISA KIT allows for the in vitro quantitative determination of Bovine Uricase, UOX concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Uricase, UOX ELISA KIT has been pre-coated with an Bovine Uricase, UOX antibody specific to Bovine Uricase, UOX .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Uricase, UOX and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Uricase, UOX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Uricase, UOX in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!