Bovine Vitamin D-binding protein, GC ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

GC,vitamin D binding protein,gc-globulin; DBP,GC; DBP; GRD3; HEL-S-51; VDBG; VDBP; VDB; epididymis secretory protein Li 51; vitamin D-binding alpha-globulin; vitamin D-binding protein; group-specific component, Gc-globulin

Search name

Bovine GC ELISA KIT ,Bovine vitamin D binding protein ELISA KIT ,Bovine gc-globulin ELISA KIT ,Bovine DBP ELISA KIT ,Bovine GC ELISA KIT ,Bovine DBP ELISA KIT ,Bovine GRD3 ELISA KIT ,Bovine HEL-S-51 ELISA KIT ,Bovine VDBG ELISA KIT ,Bovine  VDBP ELISA KIT ,Bovine VDB ELISA KIT ,Bovine epididymis secretory protein Li 51 ELISA KIT ,Bovine vitamin D-binding alpha-globulin ELISA KIT ,Bovine vitamin D-binding protein ELISA KIT ,Bovine group-specific component ELISA KIT ,Bovine  Gc-globulin ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine vitamin d-binding protein,DBP concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to DBP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DBP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DBP in the samples is then determined by comparing the O.D. of the samples to the standard curve.