Bovine Zn- Metallothionein ELISA Kit

Product Name:Bovine Zn- Metallothionein ELISA Kit
Packing:96T

Catalog No.:ELA-E0743b

Gene Name:Bovine Zn-Metallothionein

Detect Range:15.625-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Bovine Zn-Metallothionein

Alternative Name:Zn-MT,Bovine Zn-Metallothionein
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine Zn- Metallothionein ELISA Kit allows for the in vitro quantitative determination of Bovine Zn-Metallothionein concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine Zn- Metallothionein ELISA Kit has been pre-coated with an Bovine Zn-Metallothionein antibody specific to Bovine Zn-Metallothionein .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Zn-Metallothionein and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Zn-Metallothionein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Zn-Metallothionein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!