Bovine Epinephrine, EPI ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Epinephrine,Adrenaline Epinephrine,adrenaline, adrenalin,EPI,ET
Search name
Bovine Epinephrine ELISA KIT ,Bovine Adrenaline Epinephrine ELISA KIT ,Bovine adrenaline ELISA KIT ,Bovine adrenalin ELISA KIT ,Bovine EPI ELISA KIT ,Bovine ET ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Epinephrine concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epinephrine, During the reaction, Epinephrine in the sample or standard competes with a fixed amount of biotin-labeled Epinephrine for sites on a pre-coated Monoclonal antibody specific to Epinephrine. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Epinephrine in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Technical Principle & Methodology
The quality assurance and experimental performance of this biological reagent are rigorously optimized and standardized according to its specific technical pathway:
Double Antibody Sandwich Method
(双抗体夹心法). This ensures maximum reproducibility across clinical research and scientific workflows.
