Bovine Pulmonary surfactant associated protein D,PSPD ELISA Kit

Bovine Pulmonary surfactant associated protein D, PSPD ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Pulmonary surfactant-associated protein D,PSP-D,SP-D,Collectin-7,Lung surfactant protein D,surfactant protein DCOLEC7, PSPD,
SFTP4, SP-D, collectin 7, collectin-7, lung surfactant protein D, pulmonary surfactant apoprotein, pulmonary surfactant-associated protein
D, surfactant pulmonary-associated protein D, surfactant-associated protein pulmonary 4, COLEC7,CP4

Search name
Bovine Pulmonary surfactant-associated protein D ELISA KIT ,Bovine PSP-D ELISA KIT ,Bovine SP-D ELISA KIT , Bovine PSPD
ELISA KIT, Bovine Collectin-7 ELISA KIT ,Bovine Lung surfactant protein D ELISA KIT ,Bovine surfactant protein DCOLEC7
ELISA KIT , Bovine SFTP4 ELISA KIT ,Bovine SP-D ELISA KIT ,Bovine collectin 7 ELISA KIT ,Bovine collectin-7 ELISA
KIT ,Bovine lung surfactant protein D ELISA KIT ,Bovine pulmonary surfactant apoprotein ELISA KIT ,Bovine pulmonary
surfactant-associated protein D ELISA KIT ,Bovine surfactant pulmonary-associated protein D ELISA KIT ,Bovine
surfactant-associated protein pulmonary 4 ELISA KIT,Bovine COLEC7 ELISA KIT,Bovine CP4 ELISA KIT

Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine pulmonary surfactant-associated protein d,PSP-D
concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSP-D. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PSP-D and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain PSP-D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PSP-D in the samples is then determined by comparing
the O.D. of the samples to the standard curve.

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