Bovine Semaphorin- 3C,SEMA3C ELISA KIT

Bovine Semaphorin- 3C, SEMA3C ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Sema3c,SEMAE; SemE; sema E; semaphorin E; semaphorin-3C; semaphorin-E; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C

Search name

Bovine Sema3c ELISA KIT ,Bovine SEMAE ELISA KIT ,Bovine SemE ELISA KIT ,Bovine sema E ELISA KIT ,Bovine semaphorin E ELISA KIT ,Bovine semaphorin-3C ELISA KIT ,Bovine semaphorin-E ELISA KIT ,Bovine sema domain immunoglobulin domain (Ig) ELISA KIT ,Bovine short basic domain secreted ELISA KIT ,Bovine semaphorin 3C ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Bovine Semaphorin- 3C, SEMA3C concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to SEMA3C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SEMA3C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SEMA3C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SEMA3C in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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