Canine 6- phosphofructokinase, muscle type, PFKM ELISA KIT

Product Name:Canine 6- phosphofructokinase, muscle type, PFKM ELISA KIT
Packing:96T

Catalog No.:ELI-02496d

Gene Name:PFKM

Detect Range:62.5-4000 pg/mL

Sensitivity:15.6 pg/mL

Target Protein Name:Canine 6- phosphofructokinase, muscle type

Alternative Name:PFKM,Canine 6- phosphofructokinase, muscle type
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine 6- phosphofructokinase, muscle type, PFKM ELISA KIT allows for the in vitro quantitative determination ofCanine 6- phosphofructokinase, muscle type concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Canine 6- phosphofructokinase, muscle type, PFKM ELISA KIT has been pre-coated with an PFKM antibody specific to Canine 6- phosphofructokinase, muscle type .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine 6- phosphofructokinase, muscle type and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine 6- phosphofructokinase, muscle type, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine 6- phosphofructokinase, muscle type in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!