Canine Activation- induced cytidine deaminase, AICDA ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

AICDA,activation induced cytidine deaminase,AID; ARP2; CDA2; HEL-S-284; HIGM2; cytidine aminohydrolase; epididymis secretory protein Li 284; integrated into Burkitt's lymphoma cell line Ramos; single-stranded DNA cytosine deaminase; activation-induced cytidine deaminase

Search name

Canine AICDA ELISA KIT ,Canine activation induced cytidine deaminase ELISA KIT ,Canine AID ELISA KIT ,Canine ARP2 ELISA KIT ,Canine CDA2 ELISA KIT ,Canine HEL-S-284 ELISA KIT ,Canine HIGM2 ELISA KIT ,Canine cytidine aminohydrolase ELISA KIT ,Canine epididymis secretory protein Li 284 ELISA KIT ,Canine integrated into Burkitt's lymphoma cell line Ramos ELISA KIT ,Canine single-stranded DNA cytosine deaminase ELISA KIT ,Canine activation-induced cytidine deaminase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Canine Activation-induced cytidine deaminaseconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activation-induced cytidine deaminase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forActivation-induced cytidine deaminase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Activation-induced cytidine deaminase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Activation-induced cytidine deaminase in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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