Canine Allopregnanolone ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Allopregnanolone,3a,5a-tetrahydroprogesterone,3a-hydroxy-5a-pregnan-20-one,

Search name
Canine Allopregnanolone ELISA Kit, Allopregnanolone ELISA Kit, Canine 3a,5a-tetrahydroprogesterone ELISA Kit,
3a,5a-tetrahydroprogesterone ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of  allopregnanolone,Allopregnanolone concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Allopregnanolone, During the reaction, Allopregnanolone in the sample or standard competes with
a fixed amount of biotin-labeled Allopregnanolone for sites on a pre-coated Monoclonal antibody specific to Allopregnanolone. Excess
conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is
added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450
nm ± 2 nm. The concentration of Allopregnanolone in the samples is then determined by comparing the O.D. of the samples to the
standard curve.

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