Canine Alpha Hydroxybutyric acid, AHBA ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Alpha-Hydroxybutyric acid,AHBA, 2-Hydroxybutyric acid,

Search name
Canine AHBA ELISA Kit, Canine Alpha Hydroxybutyric acid ELISA Kit, Canine 2-Hydroxybutyric acid ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Canine Alpha-Hydroxybutyric acid concentrations in serum,
plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Alpha-Hydroxybutyric acid, During the reaction, Alpha-Hydroxybutyric acid in the sample or
standard competes with a fixed amount of biotin-labeled Alpha-Hydroxybutyric acid for sites on a pre-coated Monoclonal antibody specific
to Alpha-Hydroxybutyric acid. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-Hydroxybutyric acid in the samples is then
determined by comparing the O.D. of the samples to the standard curve.

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