Canine Alpha-GST ELISA Kit
Product Name:Canine Alpha-GST ELISA Kit
Gene Name:Canine Alpha-GST
Target Protein Name:Canine Alpha-GST
Alternative Name:Alpha-glutathione S-transferases,Alpha-GST
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine Alpha-GST ELISA Kit allows for the in vitro quantitative determination of Canine Alpha-GST concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine Alpha- GST ELISA Kit has been pre-coated with an Canine Alpha-GST antibody specific to Canine Alpha-GST .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Alpha-GST and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Alpha-GST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Alpha-GST in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!