Canine Beta-endorphin, BEP ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Beta-endorphin,BETA EP,beta-EP,β-EP,BEP, β endorphin, β EP

Search name

Canine Beta-endorphin ELISA KIT, Canine BETA EP ELISA KIT,, Canine beta-EP ELISA KIT, Canine,β-EP ELISA KIT,, Canine BEP ELISA KIT,, Canine β endorphin ELISA KIT, Canine β EP ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Canine beta-endorphin,Beta-endorphin concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-endorphin, During the reaction, Beta-endorphin in the sample or standard competes with a fixed amount of biotin-labeled Beta-endorphin for sites on a pre-coated Monoclonal antibody specific to Beta-endorphin. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Beta-endorphin in the samples is then determined by comparing the O.D. of the samples to the standard curve.