Canine CCKAR ELISA KIT

Product Name:Canine CCKAR ELISA KIT
Packing:96T

Catalog No.:ELI-11383d

Gene Name:Canine CCKAR

Detect Range:15.625-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Canine CCKAR

Alternative Name:Cholecystokinin receptor type A,CCKAR
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine CCKAR ELISA KIT allows for the in vitro quantitative determination of Canine CCKAR concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Canine CCKAR ELISA KIT has been pre-coated with an Canine CCKAR antibody specific to Canine CCKAR .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine CCKAR and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine CCKAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine CCKAR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!