Canine CUBN ELISA KIT

Product Name:Canine CUBN ELISA KIT
Packing:96T

Catalog No.:ELI-50630d

Gene Name:Canine CUBN

Detect Range:0.313-20ng/ml

Sensitivity:0.188ng/ml

Target Protein Name:Canine CUBN

Alternative Name:Cubilin,CUBN
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine CUBN ELISA KIT allows for the in vitro quantitative determination of Canine CUBN concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Canine CUBN ELISA KIT has been pre-coated with an Canine CUBN antibody specific to Canine CUBN .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine CUBN and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine CUBN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine CUBN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!