Canine Des ELISA Kit

Product Name:Canine Des ELISA Kit
Packing:96T

Catalog No.:ELA-E0373d

Gene Name:Canine Des

Detect Range:15.625-1000pg/ml

Sensitivity:9.375pg/ml

Target Protein Name:Canine Des

Alternative Name:desmin,Des
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Des ELISA Kit allows for the in vitro quantitative determination of Canine Des concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Canine Des ELISA Kit has been pre-coated with an Canine Des antibody specific to Canine Des .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Des and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Des, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Des in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!