Canine Dihydrotestosterone, DHT ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Dihydrotestosterone,17Beta-hydroxy-5Alpha-androstan-3-one,5Alpha-androstan-17Beta-ol-3-one,androstanolone,DHT,5Alpha-dihydrote
stosterone (5Alpha-DHT),

Search name
Canine Dihydrotestosterone ELISA Kit, Canine 17Beta-hydroxy-5Alpha-androstan-3-one ELISA Kit, Canine
5Alpha-androstan-17Beta-ol-3-one ELISA Kit, Canine androstanolone ELISA Kit, Canine 5Alpha-dihydrotestosterone ELISA Kit,
Canine DHT ELISA Kit, Canine 5Alpha-DHT ELISA Kit, Canine androstanolone ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of dihydrotestosterone,Dihydrotestosterone concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Dihydrotestosterone, During the reaction, Dihydrotestosterone in the sample or standard
competes with a fixed amount of biotin-labeled Dihydrotestosterone for sites on a pre-coated Monoclonal antibody specific to
Dihydrotestosterone. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Dihydrotestosterone in the samples is then determined by
comparing the O.D. of the samples to the standard curve.

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