Canine FUCA1 ELISA KIT
Product Name:Canine FUCA1 ELISA KIT
Packing:96T
Catalog No.:ELI-02725d
Gene Name:Canine FUCA1
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Canine FUCA1
Alternative Name:Tissue alpha-L-fucosidase,FUCA1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine FUCA1 ELISA KIT allows for the in vitro quantitative determination of Canine FUCA1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine FUCA1 ELISA KIT has been pre-coated with an Canine FUCA1 antibody specific to Canine FUCA1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine FUCA1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine FUCA1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine FUCA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!