Canine Gamma Hydroxybutyric acid, GHB ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Gamma-Hydroxybutyric acid, 4-hydroxybutyric acid, GHB,GHBA

Search name
Canine GHB ELISA Kit, Canine GHB ELISA Kit, Canine Gamma 4-hydroxybutyric acid ELISA Kit, Canine GHBA ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Canine Gamma-Hydroxybutyric acid concentrations in serum,
plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Gamma-Hydroxybutyric acid, During the reaction, Gamma-Hydroxybutyric acid in the sample or
standard competes with a fixed amount of biotin-labeled Gamma-Hydroxybutyric acid for sites on a pre-coated Monoclonal antibody
specific to Gamma-Hydroxybutyric acid. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Gamma-Hydroxybutyric acid in the samples is then
determined by comparing the O.D. of the samples to the standard curve.

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