Canine Granulocyte macrophage colony stimulating factor, GM- CSF ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Granulocyte-macrophage colony-stimulating factor,GM-CSF,Colony-stimulating factor,CSF, CSF2,GM-CSF,GMCSF; CSF; granulocyte-macrophage colony-stimulating factor; molgramostin; sargramostim; colony stimulating factor 2,granulocyte-macrophage

Search name

Canine Granulocyte-macrophage colony-stimulating factor ELISA KIT ,Canine GM-CSF ELISA KIT ,Canine Colony-stimulating factor ELISA KIT ,Canine CSF ELISA KIT ,Canine CSF2 ELISA KIT ,Canine GM-CSF ELISA KIT ,Canine GMCSF ELISA KIT ,Canine CSF ELISA KIT ,Canine granulocyte-macrophage colony-stimulating factor ELISA KIT ,Canine molgramostin ELISA KIT ,Canine sargramostim ELISA KIT ,Canine colony stimulating factor 2 ELISA KIT ,Canine granulocyte-macrophage ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Canine granulocyte-macrophage colony-stimulating factor,GM-CSF concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to GM-CSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GM-CSF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GM-CSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GM-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.