Canine HBB ELISA KIT

Product Name:Canine HBB ELISA KIT
Packing:96T                      Manual

Catalog No.:ELI-02407d

Gene Name:Canine HBB

Detect Range:0.78-50ng/ml

Sensitivity:0.39ng/ml

Target Protein Name:Canine HBB

Alternative Name:Hemoglobin subunit beta,HBB
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine HBB ELISA KIT allows for the in vitro quantitative determination of Canine HBB concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Canine HBB ELISA KIT has been pre-coated with an Canine HBB antibody specific to Canine HBB .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine HBB and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine HBB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine HBB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!