Canine Heat shock protein beta-1, HSPB1 ELISA KIT

Product Name:Canine Heat shock protein beta-1, HSPB1 ELISA KIT
Packing:96T

Catalog No.:ELI-02413d

Gene Name:Canine Heat shock protein beta-1, HSPB1

Detect Range:0.313-20ng/ml

Sensitivity:0.188ng/ml

Target Protein Name:Canine Heat shock protein beta-1, HSPB1

Alternative Name:Heat shock protein beta-1,Heat shock protein beta-1, HSPB1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Heat shock protein beta-1, HSPB1 ELISA KIT allows for the in vitro quantitative determination of Canine Heat shock protein beta-1, HSPB1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine Heat shock protein beta- 1, HSPB1 ELISA KIT
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Heat shock protein beta-1(HspB1),Heat shock 27 kDa protein(HSP 27),; HSP 27; CMT2F; HMN2B; HS.76067; HSP28; Hsp25; SRP27;
28 kDa heat shock protein; estrogen-regulated 24 kDa protein; heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock protein
beta-1; stress-responsive protein 27; heat shock 27kDa protein 1,

Search name
Canine Heat shock protein beta-1 ELISA Kit, Canine HspB1 ELISA Kit, Canine Heat shock 27 kDa protein ELISA Kit, Canine HSP
27 ELISA Kit, Canine CMT2F ELISA Kit, Canine HMN2B ELISA Kit, Canine HS.76067 ELISA Kit, Canine HSP28 ELISA Kit, Canine
Hsp25 ELISA Kit, Canine SRP27 ELISA Kit, Canine stress-responsive protein 27 ELISA Kit, Canine Heat shock 28 kDa protein
ELISA Kit, Canine Heat shock 25 kDa protein ELISA Kit, Canine estrogen-regulated 24 kDa protein ELISA Kit,

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Canine heat shock protein beta-1,HspB1 concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HspB1. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HspB1 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain HspB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HspB1 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.

has been pre-coated with an Canine Heat shock protein beta-1, HSPB1 antibody specific to Canine Heat shock protein beta-1, HSPB1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Heat shock protein beta-1, HSPB1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Heat shock protein beta-1, HSPB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Heat shock protein beta-1, HSPB1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!