Canine Leptin ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Leptin,Obesity factor, LEP,leptin (obesity homolog, mouse); leptin (murine obesity homolog); OBS; obese protein; obese, mouse,
homolog of; obesity factor

Search name
Canine Leptin ELISA Kit, Canine Obesity factor ELISA Kit,Canine Lep ELISA Kit, Canine Leptin ELISA Kit, Leptin ELISA Kit,
Obesity factor ELISA Kit, Lep ELISA Kit, Leptin ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of dog leptin,Leptin concentrations in serum, Plasma, tissue
homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leptin. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Leptin and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain Leptin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Leptin in the samples is then determined by comparing
the O.D. of the samples to the standard curve.

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