Canine Neuromedin-U-25, NMU ELISA KIT

Product Name:Canine Neuromedin-U-25, NMU ELISA KIT
Packing:96T

Catalog No.:ELI-05834d

Gene Name:Canine Neuromedin-U-25, NMU

Detect Range:0.156-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Canine Neuromedin-U-25, NMU

Alternative Name:Neuromedin-U-25,Neuromedin-U-25, NMU
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Neuromedin-U-25, NMU ELISA KIT allows for the in vitro quantitative determination of Canine Neuromedin-U-25, NMU concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine Neuromedin- U- 25, NMU ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Neuromedin-U, prepro-NMU; neuromedin-U,Nmu

 

Search name

Canine NMU ELISA Kit, Canine Neuromedin-U ELISA Kit, Canine Neuromedin U ELISA Kit, Canine prepro-NMU ELISA Kit

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Canine Neuromedin-U concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuromedin-U. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Neuromedin-U and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neuromedin-U, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Neuromedin-U in the samples is then determined by comparing the O.D. of the samples to the standard curve.

has been pre-coated with an Canine Neuromedin-U-25, NMU antibody specific to Canine Neuromedin-U-25, NMU .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Neuromedin-U-25, NMU and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Neuromedin-U-25, NMU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Neuromedin-U-25, NMU in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!