Canine RHOA ELISA KIT
Product Name:Canine RHOA ELISA KIT
Packing:96T
Catalog No.:ELI-41321d
Gene Name:Canine RHOA
Detect Range:0.313-20ng/ml
Sensitivity:0.188ng/ml
Target Protein Name:Canine RHOA
Alternative Name:Transforming protein RhoA,RHOA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine RHOA ELISA KIT allows for the in vitro quantitative determination of Canine RHOA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine RHOA ELISA KIT has been pre-coated with an Canine RHOA antibody specific to Canine RHOA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine RHOA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine RHOA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine RHOA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!