Canine Testin, TES ELISA KIT

Product Name:Canine Testin, TES ELISA KIT
Packing:96T

Catalog No.:ELI-29196d

Gene Name:Canine Testin, TES

Detect Range:0.313-20ng/ml

Sensitivity:0.188ng/ml

Target Protein Name:Canine Testin, TES

Alternative Name:Testin,Testin, TES
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Testin, TES ELISA KIT allows for the in vitro quantitative determination of Canine Testin, TES concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine Testin, TES ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

 

Alternative name

Canine TES ELISA KIT ,Canine Testin ELISA KIT ,Canine TESS ELISA KIT ,Canine TESS-2 ELISA KIT ,Canine testis derived transcript (3 LIM domains) ELISA KIT ,Canine testin LIM domain protein ELISA KIT

 

Intended use

Canine TES ELISA kit allows for the in vitro quantitative determination of Canine Testin concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

 

Test principle

Canine TES ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TES, During the reaction, TES in the sample or standard competes with a fixed amount of biotin-labeled TES for sites on a pre-coated Monoclonal antibody specific to TES. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TES in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Canine TES ELISA kit manual request by email:[email protected] 

Order Canine TES ELISA kit by email:[email protected]

has been pre-coated with an Canine Testin, TES antibody specific to Canine Testin, TES .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Testin, TES and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Testin, TES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Testin, TES in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!