Canine Trefoil factor 3, TFF3 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

TFF3, hITF,ITF; P1B; TFI; polypeptide P1.B,Trefoil factor 3,Intestinal trefoil factor,rITF,Polypeptide P1.B,rP1.B,

Search name

Canine TFF3 ELISA KIT ,Canine  hITF ELISA KIT ,Canine ITF ELISA KIT ,Canine P1B ELISA KIT ,Canine TFI ELISA KIT ,Canine polypeptide P1.B ELISA KIT ,Canine Trefoil factor 3 ELISA KIT ,Canine Intestinal trefoil factor ELISA KIT ,Canine rITF ELISA KIT ,Canine Polypeptide P1.B ELISA KIT ,Canine rP1.B ELISA KIT

Intended use

Canine TFF3 ELISA KIT allows for the in vitro quantitative determination of canine trefoil factor 3,TFF3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Canine TFF3 ELISA KIT has been pre-coated with an antibody specific to TFF3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TFF3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TFF3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TFF3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Canine TFF3 ELISA KIT Range 0.31-20ng/mL