Canine Triosephosphate isomerase, TPI1 ELISA KIT

Product Name:Canine Triosephosphate isomerase, TPI1 ELISA KIT
Packing:96T

Catalog No.:ELI-05693d

Gene Name:Canine Triosephosphate isomerase, TPI1

Detect Range:0.313-20ng/ml

Sensitivity:0.188ng/ml

Target Protein Name:Canine Triosephosphate isomerase, TPI1

Alternative Name:Triosephosphate isomerase,Triosephosphate isomerase, TPI1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Canine Triosephosphate isomerase, TPI1 ELISA KIT allows for the in vitro quantitative determination of Canine Triosephosphate isomerase, TPI1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in

Canine Triosephosphate isomerase,TPI1 ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

TPI1,triosephosphate isomerase 1; triosephosphate isomerase; HEL-S-49; TIM; TPI; TPID; epididymis secretory protein Li 49; triose-phosphate isomerase

 

Search name

Canine TPI1 ELISA KIT ,Caninetriosephosphate isomerase 1 ELISA KIT ,Canine triosephosphate isomerase ELISA KIT ,Canine HEL-S-49 ELISA KIT ,Canine TIM ELISA KIT ,Canine TPI ELISA KIT ,Canine TPID ELISA KIT ,Canine epididymis secretory protein Li 49 ELISA KIT ,Canine triose-phosphate isomerase ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Canine triosephosphate isomerase,TIM concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to TIM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TIM and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TIM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TIM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

has been pre-coated with an Canine Triosephosphate isomerase, TPI1 antibody specific to Canine Triosephosphate isomerase, TPI1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Triosephosphate isomerase, TPI1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Triosephosphate isomerase, TPI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Triosephosphate isomerase, TPI1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!