Canine Type I iodothyronine deiodinase, DIO1 ELISA KIT
Product Name:Canine Type I iodothyronine deiodinase, DIO1 ELISA KIT
Packing:96T
Catalog No.:ELI-39333d
Gene Name:Canine Type I iodothyronine deiodinase, DIO1
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Canine Type I iodothyronine deiodinase, DIO1
Alternative Name:Type I iodothyronine deiodinase,Type I iodothyronine deiodinase, DIO1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Canine Type I iodothyronine deiodinase, DIO1 ELISA KIT allows for the in vitro quantitative determination of Canine Type I iodothyronine deiodinase, DIO1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Canine Type I iodothyronine deiodinase, DIO1 ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
DIO1,5DI; TXDI1; DIOI; thyroxine deiodinase type I selenoprotein; type 1 DI; type I iodothyronine deiodinase; type-I 5'-deiodinase; deiodinase iodothyronine, type I ,Type I iodothyronine deiodinase,Type-I 5'-deiodinase,Type 1 DI,5DI,DIOI,
Search name
Canine DIO1 ELISA KIT ,Canine 5DI ELISA KIT ,Canine TXDI1 ELISA KIT ,Canine DIOI ELISA KIT ,Canine thyroxine deiodinase type I selenoprotein ELISA KIT ,Canine type 1 DI ELISA KIT ,Canine type I iodothyronine deiodinase ELISA KIT ,Canine type-I 5'-deiodinase ELISA KIT ,Canine deiodinase iodothyronine type I ELISA KIT ,Canine Type I iodothyronine deiodinase ELISA KIT ,Canine Type-I 5'-deiodinase ELISA KIT ,Canine Type 1 DI ELISA KIT ,Canine 5DI ELISA KIT ,Canine DIOI ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Canine type Type I iodothyronine deiodinase concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Type I iodothyronine deiodinase, During the reaction, Type I iodothyronine deiodinase in the sample or standard competes with a fixed amount of biotin-labeled Type I iodothyronine deiodinase for sites on a pre-coated Monoclonal antibody specific to Type I iodothyronine deiodinase. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Type I iodothyronine deiodinase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
has been pre-coated with an Canine Type I iodothyronine deiodinase, DIO1 antibody specific to Canine Type I iodothyronine deiodinase, DIO1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Canine Type I iodothyronine deiodinase, DIO1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Canine Type I iodothyronine deiodinase, DIO1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Canine Type I iodothyronine deiodinase, DIO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!