Canine Vitamin B12, VB12 ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Vitamin B12,cobalamin,vitamin B-12,vb12

Search name

Canine Vitamin B12 ELISA KIT ,Canine cobalamin ELISA KIT ,Canine vitamin B-12 ELISA KIT ,Canine vb12 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Canine Vitamin B12 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin B12, During the reaction, Vitamin B12 in the sample or standard competes with a fixed amount of biotin-labeled Vitamin B12 for sites on a pre-coated Monoclonal antibody specific to Vitamin B12. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Vitamin B12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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