BL21(DE3) chemically Competent Cells

BL21(DE3):                                       10×100μl

pUC19(control vector):  10pg/μl        

Store at –80°C

Genotype  

F- ompT hsdSB(rB- mB- ) gal dcm(DE3)

Alternative name
BL21 (DE3) chemically Competent Cells,BL21 (DE3) Competent E.coli Express Competent Cells, BL21 (DE3) chemically E.coli Express Express Competent Cells 

Transformation Protocol

1.   Inoculate a single colony from a freshly streaked plate into 5 ml of LB medium containing the appropriate antibiotic for the plasmid and host strain.

2.   Incubate with shaking at 200 rpm at 37