Chicken beta Hydroxybutyric acid, BHBA ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
beta-Hydroxybutyric acid,beta-hydroxybutyrate,3-hydroxybutyric acid,BHBA,3-hydroxybutyrate

Search name
Chicken beta Hydroxybutyric acid ELISA Kit, Chicken BHBA ELISA Kit, Chicken beta-Hydroxybutyric acid ELISA Kit, Chicken
beta-hydroxybutyrate ELISA Kit, Chicken 3-hydroxybutyric acid ELISA Kit, Chicken 3-hydroxybutyrate ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Chicken beta-hydroxybutyric acid,beta-Hydroxybutyric acid
concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to beta-Hydroxybutyric acid, During the reaction, beta-Hydroxybutyric acid in the sample or standard
competes with a fixed amount of biotin-labeled beta-Hydroxybutyric acid for sites on a pre-coated Monoclonal antibody specific to
beta-Hydroxybutyric acid. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of beta-Hydroxybutyric acid in the samples is then
determined by comparing the O.D. of the samples to the standard curve.

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