Chicken Calcitonin, CALCA ELISA Kit
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Calcitonin,thyrocalcitonin,CALCA; CALC1; CGRP; CGRP-I; CGRP1; CT; KC, Calcitonin,alpha-type CGRP; CGRP-I; CGRP; CALC1;
CGRP1; Calcitonin 1; calcitonin gene-related peptide I; calcitonin/calcitonin-related polypeptide, alpha; katacalcin; calcitonin-related
polypeptide alpha

Search name
Chicken Calcitonin ELISA Kit, Chicken calcitonin gene related peptide ELISA Kit, Chicken CGRP ELISA Kit, Chicken
thyrocalcitonin ELISA Kit, Chicken CALCA ELISA Kit, Chicken CALC1 ELISA Kit, Chicken CGRP-I ELISA Kit, Chicken CGRP1
ELISA Kit, Chicken CT ELISA Kit, Chicken KC ELISA Kit, Chicken Calcitonin,alpha-type CGRP ELISA Kit, Chicken Calcitonin 1
ELISA Kit, Chicken calcitonin gene-related peptide I ELISA Kit, Chicken calcitonin/calcitonin-related polypeptide alpha ELISA
Kit, Chicken katacalcin ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of chicken Calcitonin concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.

Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Calcitonin, During the reaction, Calcitonin in the sample or standard competes with a fixed amount
of biotin-labeled Calcitonin for sites on a pre-coated Monoclonal antibody specific to Calcitonin. Excess conjugate and unbound sample or
standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Calcitonin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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