Chicken Desmopressin, dDAVP ELISA Kit
FOR RESEARCH USE ONLY!
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Chicken dDAVP ELISA kit allows for the in vitro quantitative determination of Chicken Desmopressin concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Chicken dDAVP ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to dDAVP, During the reaction, dDAVP in the sample or standard competes with a fixed amount of biotin-labeled dDAVP for sites on a pre-coated Monoclonal antibody specific to dDAVP. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of dDAVP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Chicken dDAVP ELISA kit Range 6.25-400pg/ml
Chicken dDAVP ELISA kit manual download
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