Chicken Fibulin-1, FBLN1 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Fibulin-1,FIBL-1, FBLN,FIBL1,fibulin-1,fibulin 1,FBLN1, PP213

Search name

Chicken Fibulin-1 ELISA KIT ,Chicken FIBL-1 ELISA KIT ,Chicken FBLN ELISA KIT ,Chicken FIBL1 ELISA KIT ,Chicken fibulin-1 ELISA KIT ,Chicken fibulin 1 ELISA KIT ,Chicken FBLN1 ELISA KIT, Chicken PP213 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Chicken fibulin-1,FIBL-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to FIBL-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FIBL-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FIBL-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FIBL-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.